Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) |
OL0070B | https://doi.org/10.7554/eLife.21022.001 | OL0070B | LC6 split-GAL4 driver line available via https://www.janelia.org/split-GAL4. |
| Genetic reagent (D. melanogaster) |
OL0081B | https://doi.org/10.7554/eLife.21022.001 | OL0081B | LC6 split-GAL4 driver line available via https://www.janelia.org/split-GAL4. |
| Genetic reagent (D. melanogaster) |
SS00825 | This paper | SS00825 | LG6G1 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4. |
| Genetic reagent (D. melanogaster) |
SS00824 | This paper | SS00824 | LG6G1 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS02036 | This paper | SS02036 | LG6G2 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS02099 | This paper | SS02099 | LG6G3 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS02699 | This paper | SS02699 | LG6G3 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS03690 | This paper | SS03690 | LG6G4 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS02410 | This paper | SS02410 | LG6G5 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS02409 | This paper | SS02409 | LG6G5 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS25111 | This paper | SS25111 | LG6G5 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
SS03641 | This paper | SS03641 | LG26G1 split-GAL4 driver line, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) |
pJFRC51-3XUAS-IVS-Syt::smHA in su(Hw)attP1, pJFRC225-5XUAS-IVS-myr::smFLAG in VK00005 | https://doi.org/10.1073/pnas.1506763112 | Presynaptic and membrane marker | |
| Genetic reagent (D. melanogaster) |
MCFO-1: pBPhsFlp2::PEST in attP3;; pJFRC201-10XUAS-FRT > STOP > FRT-myr::smGFP-HA in VK0005, pJFRC240-10X-UAS-FRT>STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT > STOP > FRT-myr::smGFP-FLAG in su(Hw)attP1 | https://doi.org/10.1073/pnas.1506763112 | RRID:BDSC_64085 | MultiColor FlipOut (stochastic labeling) |
| Genetic reagent (D. melanogaster) |
42E06- LexA (JK22C) | https://doi.org/10.1534/genetics.114.173187 | ||
| Genetic reagent (D. melanogaster) |
13XLexAop2-CsChrimson-tdT (attP18), 20XUAS-IVS-Syn21-opGCaMP6f p10 (Su(Hw)attp8) | https://elifesciences.org/articles/49257/ and this paper | Functional connectivity | |
| Genetic reagent (D. melanogaster) |
pJFRC7-20XUAS-IVS- GCaMP6m (VK00005) |
10.1038/s41592-019-0435-6 | RRID:BDSC_52869 | |
| Genetic reagent (D. melanogaster) |
pJFRC48-13XLexAop2-IVS-myrtdTomato (su(Hw)attP8) | This paper | ||
| Genetic reagent (D. melanogaster) |
UAS-7xHaloTag::CAAX (VK00005) | https://doi.org/10.1534/genetics.116.199281 | HaloTag reporter used for FISH | |
| Sequence-based reagent | FISH probes for GAD1, VGlut and ChaT | https://doi.org/10.1038/nmeth.4309 | ||
| Antibody | Anti-HA rabbit monoclonal C29F4 | Cell Signaling Technologies: 3724S | RRID:AB_1549585 | (1:300) |
| Antibody | Anti-FLAG rat monoclonal DYKDDDDK Epitope Tag Antibody [L5] | Novus Biologicals: NBP1-06712 | RRID:AB_1625981 | (1:200) |
| Antibody | DyLight 550 conjugated anti-V5 mouse monoclonal | AbD Serotec: MCA1360D550GA | RRID:AB_2687576 | (1:500) |
| Antibody | DyLight 549 conjugated anti-V5 mouse monoclonal | AbD Serotec: MCA1360D549GA | RRID:AB_10850329 | (1:500) |
| Antibody | Anti-Brp mouse monoclonal nc82 | DSHB | RRID:AB_2314866 | (1:30) |
| Software, algorithm | Python 3.7.8 | http://www.python.org | RRID:SCR_008394 | |
| Software, algorithm | neuprint python | https://github.com/connectome-neuprint/neuprint-python | ||
| Software, algorithm | FluoRender | http://www.sci.utah.edu/software/fluorender.html | RRID:SCR_014303 | |
| Software, algorithm | VVD_Viewer | https://github.com/takashi310/VVD_Viewer | ||
| Software, algorithm | Fiji | http://fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | R | https://www.r-project.org/ | Anatomical analysis | |
| Software, algorithm | R studio | http://www.rstudio.com/ | ||
| Software, algorithm | Natverse package in R | http://natverse.org | EM anatomy tools | |
| Software, algorithm | MATLAB | Mathworks, Inc | Multiple versions 2016–2019 | Visual stimuli, experiment control, data analysis, and eye alignment |
| Software, algorithm | Nia (neuron image analysis) | https://bitbucket.org/jastrother/neuron_image_analysis | ||
| Software, algorithm | LED Panel display tools |
https://github.com/reiserlab/Modular-LED-Display
(Generation 3) |
||
| Software, algorithm | Thunder-project | https://github.com/thunder-project/thunder | http://thunder-project.org/ image registration – functional connectivity |