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Schematic comparison of three different sandwich ELISA formats for human sEH detection using the same horseradish peroxidase (HRP) labeled anti-sEH nanobody conjugate (HRP-A1) as detection antibody. (a) format A (pAb/HRP-A1): polyclonal antibody passively adsorbed, (b) format B: (A9/HRP-A1): nanobody passively adsorbed, (c) format C (SA/biotin-A9/HRP-A1): nanobody biotinylated and bound to streptavidin passively adsorbed, as capture antibody, respectively.
