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Schematic preparation of the enzyme HRP labeled nanobody A1 conjugate (HRP-A1) based on a mild periodate oxidation (Malaprade reaction). HRP is a heme-containing glycoprotein that may be oxidized with sodium periodate. The resulting reactive aldehyde residues may be conjugated to nanobody with Schiff base formed and further stabilized by sodium cyanoborohydride.
