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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: Anal Chem. 2020 Apr 27;92(10):7334–7342. doi: 10.1021/acs.analchem.0c01115

Figure 4.

Figure 4.

Calibration curves of SBdNb ELISAs (format C) with varying concentrations of detection antibody (HRP-A1, 1:1600–1:12800 dilution) in PBS (a) or 10% SM/PBS (b). Other conditions: streptavidin, 2.5 μg/mL coated in CB overnight at 4 °C; blocking buffer, 2% BSA/PBS 1 h; biotin-A9, 1.25 μg/mL 1 h; HRP-A1, 1h; TMB 15 min. All same or similar steps were performed under the same conditions (e.g., incubation time, reagents). Error bars indicate standard deviations (n = 3). All coefficient of determination R2>0.99.