Figure 2.
Impact of Subdoligranulum variabile supplementation on the prevention of diet-induced obesity in mice
(A) Study design: Mice were fed a high-fat diet (HFD) and gavaged with 1.5 × 109 cultivable cells (cc) of freshly prepared life bacteria, 5 days a week for 8 weeks (HFD-Subdo group, n = 10, blue). This group was compared to mice that were gavaged with vehicle and were fed either a control diet (Control group, n = 11, green) or a high-fat diet (HFD group, n = 10, black). (B) Body weight (g) (C) lean mass (g) (D) and fat mass (g) evolution during the 8 weeks follow-up. (E) Cumulative food intake in kcal during the 8 weeks of treatment. (F) Weight in mg of different fat depots at the end of the experiment. SAT: subcutaneous (inguinal), EAT: epididymal, VAT: visceral (mesenteric), BAT: brown adipose tissue. (G) Weight in mg of different types of muscles. (H) qPCR quantification of Subdoligranulum at the end of a 5-day period of gavage. Data were analyzed using one-way ANOVA followed by a Tukey post hoc test for E-H or using 2-way repeated measures ANOVA for B-C. ‘*’ ‘**’ and ‘***’ indicate a significant difference versus HFD (P < .05, P < .01, P < .001, respectively).