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Effects of TRIF deficiency on bodyweight, food consumption, and plasma leptin levels. (a) Animals were weighed weekly from 16 weeks of age through 44 weeks. (b) Food consumption was recorded weekly from 36 weeks through 40 weeks. (c) Plasma samples were harvested at the age of 48 weeks, and plasma leptin was determined by leptin ELISA kit ( ∗P < 0.05, ##P < 0.001) (n = 12/group).
