Figure 4.

Effects of TRIF deficiency on activation of adipose tissue and liver AKT by insulin administration in mice. At 48 weeks of age, mice were fasted for 16 h and then intraperitoneally administered with PBS (a, c, e) or insulin (5 U/kg bodyweight) (b, d, f). Epididymal adipose tissue, muscle, and liver were collected 10 min after the administration. Levels of phospho-AKT (S473, P-Akt) and total-AKT (T-AKT) in adipose tissue (a, b), muscle (c, d), and liver (e, f) were determined by western blotting. (g–i) Bar graphs represent the results of densitometric analysis of western blots shown in (a) and (b), in (c) and (d), and in (e) and (f), respectively ( ^∗P < 0.05,  ^##P < 0.001) (n = 4/group).