Fig. 2.

Reg4 levels are correlated with intestinal inflammation. a Quantification of Reg4 mRNA in mouse colon with or without DSS treatment using RT-PCR assay. b Colorimetric in situ hybridization (CISH) analysis for Reg4 in mouse colon. c The Reg4 protein levels were determined in mouse colon using western blot. d–f The levels of serum Reg4, IL-6 and TNF-α were determined in healthy controls (HC, n = 16) and children with intestinal failure (IF, n = 40) using the enzyme linked immunosorbent assay (ELISA). (g, h) The serum Reg4 levels were correlated with serum IL-6 and TNF-α. **p < 0.01, ***p <0.001