Fig. 4.

ATF2 promotes Reg4 transcription in during the intestinal inflammation. a Western blot analysis for p-ATF2 and ATF2 in the colonic mucosa with or without DSS treatment. b Representative images of the immunohistochemistry (IHC) staining of p-ATF2. c The binding motif of ATF2 on the promoter of Reg4. a The binding motif of ATF2 on the promoter of Reg4. c–f The promoter activity, mRNA and protein of Reg4 were reduced by ATF2 knockdown. C, The promoter activity was analyzed by luciferase reporter system. D, The mRNA levels were determined by RT-PCR. E, Western blot analysis for Reg4 and ATF2. F, Immunofluorescence staining for Reg4 and ZO1. *p < 0.05, **p < 0.01