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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Nat Metab. 2020 Nov 30;2(12):1401–1412. doi: 10.1038/s42255-020-00316-0

Extended Data Fig. 6. KL cells are sensitive to inhibition of GFPT activity.

Extended Data Fig. 6

a, Effect of azaserine treatment (1μM) on K and KL cells’ viability (14 cell lines, n=6). Data are the average and SD of six independent cultures. b, Effect of azaserine treatment on cell death in K and KL cells (n=3). c, Relative viability of shGFP and shLKB1-expressing H1373 following a 72hr exposure to azaserine (1μM). d, Effect of azaserine treatment on global O-GlcNAcylation of K and KL cells. Actin is used as a loading control. Statistical significance was assessed using Student’s t-tests (a) and one-way ANOVA followed by Tukey’s multiple comparisons test (c). In c, *, p<0.05 comparing to shGFP without azaserine treatment; $, p<0.05 comparing to shGFP with azaserine treatment; #, p<0.05 comparing to shLKB1 without azaserine treatment. ****, p<0.0001. Cell viability assay and Western blots were repeated at least twice. FACS analysis was performed once.