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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Nat Metab. 2020 Nov 30;2(12):1401–1412. doi: 10.1038/s42255-020-00316-0

Extended Data Fig. 8. KL cells are sensitive to inhibition of GFPT2 (cont.).

Extended Data Fig. 8

a and b, Abundance of GlcNAc-6-P, ManNAc and GlcNAc-6-P in Dox-inducible NC KO H460 (a) and H157 (b) cells (n=3). c, Abundance of GFPT2 protein in EV and LKB1-expressing H460 transfected with a control esiRNA or esiRNA directed against GFPT2. Actin is used as a loading control. d, Left, Relative viability of EV and LKB1-expressing H2122 cells after GFPT2 silencing for 96hr. Right, Abundance of GFPT2 protein in EV and LKB1-expressing H2122 cells transfected with a control esiRNA or esiRNA directed against GFPT2. Actin is used as a loading control. e, Relative viability of EV and LKB1-expressing H460 (Left) and H2122 (Right) cells after GFPT1 silencing for 96hr. f, Abundance of GFPT1 protein in EV and LKB1-expressing H2122 cells transfected with a control esiRNA or esiRNA directed against GFPT1. Actin is used as a loading control. g, Abundance of GFPT2 protein in EV and constitutively active AMPK (CA AMPK)-expressing H460 cells transfected with a control esiRNA or esiRNA directed against GFPT2. Actin is used as a loading control.