Table 3.

Expression-fold changes in targeted genes driving skeletal phenotype in 23-week old males (n = 8).

Gene family	Gene	qPCR expression	RNAseq expression
Bone
^†Fgf activity	Fgf23	+6.0^**	+2.8^**
^†Wnt activity	Lrp6	−2.5^*	−1.2
	Wnt5a	−2.8^*	−2.1
	Ctnnb1	−1.8^*	−1.1
^†Collagens	Col1a1	−3.3	−1.5
^†Metallopeptidases	Mmp2	−2.5^*	−1.5
^†Purinergic	Alpl	−2.8^*	−1.4
	Abcc6	+6.9^**	Below detection limit
	Cd73	−2.4^**	Below detection limit
^†Bone formation	Bglap (OCN)	−2.6^**	−1.3
	Sp7	−1.8^**	−1.4
	Ibsp (BSP)	−2.1^*	−1.3
^†Bone resorption	Tnfrsf11b	−4.6^*	−1.1
Liver
^‡Wnt activity	Sfrp1	+2.1^*	N/A
^‡Wnt activity	Fzd8	−3.4^**	N/A
Kidney
^‡Wnt activity	Wif1	+11.7^*	N/A

^†

Comparison of fold-changes by RT-qPCR and RNAseq.

^‡

RT-qPCR evaluation of systemic Wnt activity in select organs.

p < 0.05,

^**

p < 0.01,

Student’s unpaired t-test. The following genes were tested in 23-week old males, and demonstrated no statistically significant differences: Sost, Wisp2, Lrp5, Csnk1a1, Ctnnb1, Apc, Axin1, Dvl1, and Gsk3b.

Blue cells denote organs from which RNA was extracted and gene expression measured.