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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Curr Opin Biotechnol. 2020 Sep 18;66:217–226. doi: 10.1016/j.copbio.2020.08.005

Figure 2. Representative screening methods used to facilitate the directed evolution of oxidoreductases.

Figure 2

PECAN (probing enzymes with click-assisted NIMS), NpCN (4-nitrophenylacetonitrile), NESPA (NAD(P)-eliminated solid-phase assay), soxR (Redox-sensitive transcriptional activator), FACS (Fluorescence Activated Cell Sorting), HRP (Horse radish peroxidase), and FADS (Fluorescence Activated Droplet Sorting). Targeted Oxidoreductases: P450BM3 (NADPH-dependent Cytochrome P450 BM3), Tm 6PGDH (NADP+-dependent Thermotoga maritima 6-phosphogluconate dehydrogenase), Ld ldh (NADH-dependent Lactobacillus delbrueckii d-lactate dehydrogenase), Lb adh (NADPH-dependent Lactobacillus brevi alcohol dehydrogenase), Bo CHAO (FADH2-dependent Brevibacterium oxydans cyclohexamine oxidase)