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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Dig Dis Sci. 2020 Jun 17;66(7):2250–2260. doi: 10.1007/s10620-020-06389-x

Figure 2. Effect of EPEC on hSVCT1 and hSVCT2 protein and mRNA levels.

Figure 2.

Infecting Caco-2 cells monolayers with EPEC (WT) and HS4 (100 MOI) for 60 min with additional 6 h post incubation after EPEC removal and subjected to Western blot and RT-qPCR analyses as described in “Methods”. The hSVCT1 (A) and hSVCT2 (B) levels of expression were measured by Western blotting using protein isolated from EPEC (WT), HS4, and uninfected control Caco-2 cells. The specificity of hSVCT1 and hSVCT2 specific bands were determined by competing hSVCT1 and hSVCT2 polyclonal antibodies with synthetic antigenic peptides generated against hSVCT1 and hSVCT2 polypeptides (Ci, ii). The mRNA levels of hSVCT1 (D) and hSVCT2 (E) were determined in infected and uninfected control Caco-2 cells by RT-qPCR. Values are mean ± SE of at least triplicate determinations from different passages of cells. ** P < 0.05; * P < 0.01.