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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Dig Dis Sci. 2020 Jun 17;66(7):2250–2260. doi: 10.1007/s10620-020-06389-x

Figure 3. Effect of acute and neonatal exposure of mice to EPEC infection on 14C-AA uptake, mSVCT1 and mSVCT2 expression levels in mouse jejunum.

Figure 3.

14C-AA uptake was measured in mouse jejunum after acute exposure of mice with EPEC (WT) infection (see “Methods”) (A). Western blot was done using 60 μg of protein obtained from both acute and neonatal exposed jejunum mucosa in order to measure the mSVCT1 (B, G) and mSVCT2 (C, H) protein levels. The specificity of mSVCT1 and mSVCT2 specific bands were determined by competing with mSVCT1 and mSVCT2 polyclonal antibodies against synthetic antigenic peptides (Di, ii). The mSVCT1 (E, I) and mSVCT2 (F, J) mRNA expression levels in mouse jejunum were determined by RT-qPCR. Values are mean ± SE of at least triplicate determinations from different mice. ** P < 0.05; * P < 0.01.