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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Dig Dis Sci. 2020 Jun 17;66(7):2250–2260. doi: 10.1007/s10620-020-06389-x

Figure 4. Effect of acute and neonatal exposure of mice to EPEC infection on 14C-AA uptake, mSVCT1 and mSVCT2 expression levels in mouse colon.

Figure 4.

14C-AA uptake was determined in mouse colon after acute exposure of EPEC (WT) infection (see “Methods”) (A). Western blot was conducted using 60 μg of protein obtained from both acute and neonatal exposed colon mucosa in order to assess the mSVCT1 (B, F) and mSVCT2 (C, G) protein levels. The mSVCT1 (D, H) and mSVCT2 (E, I) mRNA expression levels in mouse colon were measured using RT-qPCR. Data are mean ± SE of at least triplicate determinations from different mice. ** P < 0.05.