Skip to main content
. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Cancer Res. 2020 Sep 30;80(24):5531–5542. doi: 10.1158/0008-5472.CAN-20-0503

Figure 3. Effects of IR and Hedgehog activation on SMG macrophages.

Figure 3.

A-B: IPA of RNA-seq data related to macrophages. Z-scores were shown in the heat maps. Z-scores ≥2 or ≤−2 are considered as significantly activated or inhibited respectively. N = 3.

C-E: Flow cytometric analyses on F4/80+/Ly6clow SG-resident macrophages (rMϕ) and F4/80+/Ly6chigh infiltrating macrophages (iMϕ) (C) and the related quantification data (D-E). D3: day 3; D7: day 7.

F: qRT-PCR assay on mRNA expression of pan-macrophage markers (Aif1, Axl, Cd11c, F4/80, Fcgr1) and rMϕ marker Cx3cr1 in SMGs.

G-H: ELISA on protein expression of pan-macrophage marker Aif1 and rMϕ marker Cx3cr1 in the lysate of SMGs.

N = 5 in C-H, ^: P < 0.05 vs. NT; *: P < 0.05 vs. IR D7.