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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Cancer Res. 2020 Sep 30;80(24):5531–5542. doi: 10.1158/0008-5472.CAN-20-0503

Figure 4. Effects of depleting SMG macrophages on Hedgehog-mediated rescue of IR-impaired salivary function.

Figure 4.

A: The experiment design.

B-D: SMGs were collected 7 days after IR or 3 days after CL treatment, and analyzed with flow cytometric for F4/80+/Ly6clow SG-resident macrophages and F4/80+/Ly6chigh infiltrating macrophages.

E: The pilocarpine-stimulated whole saliva flow rates were measured at Day 90 after IR and normalized to that in NT group.

F: The mRNA level of Aqp5 in mouse SMGs collected 90 days after IR was examined by qRT-PCR.

G: The protein level of Aqp5 in mouse SMGs collected 90 days after IR was examined by western blot and normalized with corresponding Gapdh levels.

N = 5, *: P < 0.05, ns: not significant.