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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: Immunity. 2020 Oct 20;53(6):1202–1214.e6. doi: 10.1016/j.immuni.2020.10.002

Fig. 2. Treg cell-specific Tgfb1 haploinsufficiency promotes allergic dysregulation.

Fig. 2.

(A) Core body temperature drop following OVA challenge of OVA/SEB sensitized WT, Il4raF709 and Foxp3YFPcreTgfb1Δ/+ mice. (B) Serum total and OVA-specific IgE and MMCP-1, mast cell counts from the histology of the small intestinal (C) Microscopic pictures (original magnification; x600) of toluidine blue stained histological sections from the jejunum of mice sensitized and challenged in (A). Arrows indicate mast cells. (D) Flow cytometric analysis of mast cells in the SI-LPL of mice challenged in (A). (E, G) Flow cytometric analysis and frequencies of Helios+ and Helios− Treg cells (E) IL-4+ (F) and GATA-3+ (G) Treg and Teff cells in SI-LPL of mice from panel (A). Each symbol represents an independent sample. Numbers in flow plots indicate percentages. Error bars indicate SEM. Statistical tests: repeat measure two-way ANOVA (A); One-way ANOVA with Dunnett’s post hoc analysis (B, E, F). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data representative of at least three independent experiments with 9–31 mice per group for (A) and 5–17 mice per group for panels (B, E, F, G). *p<0.05, **p<0.01 and p****<0.0001.