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. 2020 Dec 3;11:587663. doi: 10.3389/fphar.2020.587663

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Copyright © 2020 Wang, Long, Huang, Zhang, Xie, Chen, Liu, Xiong and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of BSHX decoction on nephrin, podocin, FSP-1, and α-SMA levels in DN mouse kidney. (A) IHC staining of the kidney (×400) to analyze the expression levels of nephrin, podocin, FSP-1, and α-SMA. (B) Western blot assays to evaluate the expression levels of nephrin, podocin, FSP-1 and α-SMA. Beta-actin was measured as the loading control. (C) Quantitative analysis of the relative expression levels of nephrin. (D) Quantitative analysis of the relative expression levels of podocin. (E) Quantitative analysis of the relative expression levels of FSP-1. (F) Quantitative analysis of the relative expression levels of α-SMA. n = 5 mice per group. All data are expressed as means ± SEM. ^* p < 0.05, ^* ^* p < 0.01, ^* ^* ^* p < 0.001 vs. the NC group; ^# p < 0.05, ^## p < 0.05, ^### p < 0.001 vs. the DN model group.