TABLE 1.
Characterization of the polyphenol profile of C. lumia juice by RP-LC-DAD-ESI-MS analysis.
| Peak no. | Compound | RT | Area % | λmax | m/z | [M-H]-/[M-H]+ |
|---|---|---|---|---|---|---|
| Hydroxybenzoic acids | ||||||
| 5 | 1,1′-biphenyl, 4-Hydroxybenzoic acid | 14.70 | 1.50 ± 0.08 | 306 | 292 | /293 |
| Hydroxycinnamic acids | ||||||
| 2 | Feruloyl-O-sinapoyl-O-caffeoylquinic acid | 9.61 | 0.07 ± 0.00 | 280 | 736 | 735/737 |
| 3 | (E)-3-(3,4-diacetoxy-5-methoxyphenyl)-acroyl-4-O-p-Coumaroyl-5-O-Caffeoylquinic acid | 12.59 | 1.69 ± 0.02 | 302 | 778 | 777/ |
| 7 | Caffeoylquinate shikmate derivative | 18.14 | 0.13 ± 0.01 | 270 | 510 | 509/511 |
| 18 | 1-Sinapoyl-2-feruloylgentiobiose | 39.74 | 1.31 ± 0.06 | 282; 350 | 724 | 723/725 |
| 22 | 1-Caffeoyl-5-feruloylquinic acid | 49.80 | 25.70 ± 1.55 | 284; 336 | 530 | 531/529 |
| 26 | 3,4-Dicaffeoylquinic acid | 60.66 | 0.78 ± 0.01 | 280; 336 | 516 | 515/ |
| Flavonols | ||||||
| 4 | Kaempferol 3,7,4′-O-triglucoside | 13.79 | 0.07 ± 0.00 | 304 | 772 | 771/773 |
| 8 | Limocitrol-O-glucoside | 18.79 | 0.23 ± 0.00 | 286; 328 | 538 | 537/539 |
| 17 | Quercetin 3-(2''-p-hydroxybenzoyl-4''-p-Coumarylrhamnoside) | 35.47 | 6.77 ± 0.15 | 290; 330 | 714 | 713/ |
| 19 | Quercetin 3-O-(6"-malonyl-glucoside)-7-O-glucoside | 40.62 | 0.11 ± 0.01 | 282; 350 | 712 | 711/713 |
| 21 | Kaempferol 3′- rhamnoside | 47.80 | 23.12 ± 0.84 | 272; 348 | 432 | 431/432 |
| 24 | Quercetin 3-O-(6"-malonyl-glucoside) | 57.68 | 7.72 ± 0.38 | 280; 336 | 550 | 549/551 |
| 25 | Dihydroquercetin 3-O-rhamnoside | 59.54 | 4.41 ± 0.18 | 284; 316 | 449 | 448/450 |
| 28 | Isorhamnetin 3(7)-O-glucuronopyranosyl-(1-2)-O-glucuronopyranoside | 62.62 | 0.94 ± 0.04 | 284; 326 | 668 | 669/667 |
| Flavones | ||||||
| 1 | Apigenin 7-O-glucuronide | 6.61 | 0.57 ± 0.02 | 280; 300 | 446 | 445/447 |
| 9 | Apigenina-7-O-glucoside | 19.47 | 1.56 ± 0.03 | 280; 328 | 432 | 431/433 |
| 6 | Myricetin 3-O-galactoside | 15.83 | 0.08 ± 0.00 | 274 | 480 | 479/481 |
| 13 | Diosmetin-6-C-b-D-glucoside | 29.43 | 9.35 ± 0.25 | 260; 300 | 462 | 461/463 |
| 14 | Diosmetin 6,8-di-C-glucoside | 30.85 | 2.04 ± 0.08 | 260; 300 | 624 | 623/625 |
| 23 | Tricin 7-O-[glucuronopyranosyl-(1-2)-O-methyloglucuronopyranoside] | 54.54 | 0.10 ± 0.01 | 276; 336; 370 | 696 | 695/697 |
| 27 | Orientin-sulphate | 61.56 | 1.14 ± 0.07 | 280; 336; 370 | 528 | 527/529 |
| Flavanones | ||||||
| 10 | Neohesperidin dihydrochalcone | 21.96 | 4.51 ± 0.08 | 260; 296 | 612 | 611/613 |
| 11 | Naringenin-7-O-glucoside | 23.34 | 1.19 ± 0.03 | 284; 325; 370 | 433 | 432/434 |
| 15 | Neoeriocitrin | 32.89 | 0.05 ± 0.00 | 286; 326 | 596 | 595/597 |
| 16 | Neohesperidin | 34.35 | 1.68 ± 0.06 | 274; 334 | 610 | 609/611 |
| 12 | 5,7-dihydroxy-49-methoxyflavanone 7-O-Neohesperidoside | 28.57 | 1.97 ± 0.03 | 280; 330 | 594 | 593/595 |
| Isoflavones | ||||||
| 20 | 6''-O-Malonyl daidzin | 45.81 | 1.21 ± 0.05 | 280; 338 | 502 | 501/503 |
Results were expressed as mean area percentage ± standard deviation (n = 3) of each compound with respect to the total area of polyphenols identified. Bold numbers refer to the most abundant compounds.