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. 2020 Dec 3;11:578009. doi: 10.3389/fmicb.2020.578009

FIGURE 1.

FIGURE 1

Purification of CPSIT_P7 protein and cytokine level analysis. (A) SDS-PAGE analysis of recombinant protein CPSIT_P7 purification. Lane M, pre-stained protein marker; Lane 1: flow through; Lanes 2–7: 10, 20, 30, 50, and 50 mM imidazole washing buffer, respectively. Lanes 8–12: 100, 150, 150, 250, and 250 mM imidazole eluting buffer, respectively. The recombinant CPSIT_P7 protein with a predicted size of 28 kDa. (B) THP-1 cells were stimulated with PBS (control) or CPSIT_P7 (10.0 μg/ml, 12 h). Culture supernatants were collected, pooled (n = 3/group), and screened for inflammatory cytokine, and then detected by a human cytokine antibody array. Signals were quantified and the cytokines are listed. IL-6 and IL-8: interleukin 6 and 8; MCP-1: monocyte chemotactic protein 1.