FIGURE 10.

The NF-κB signaling pathway is essential for CPSIT_P7-induced IL-6, IL-8, and MCP-1 expression. THP-1 cells were pretreated with 30 μM IκBα inhibitor BAY11-7082 for 30 min and then stimulated with 10 μg/ml CPSIT_P7 for 24 h or 36 h. (A,B) RT-qPCR and ELISA were used to detect the IL-6, IL-8, and MCP-1 mRNA expression levels and IL-6, IL-8, and MCP-1 protein production. (C) THP-1 cells were stimulated with CPSIT_P7 (10.0 μg/ml, 6 h). Cells were stained with anti-NF-κB p65 antibody, followed by fluorescein-labeled secondary antibody incubation. Images were procured using a confocal microscope (10 × 100). Data are the means ± SD of three independent experiments. ^#P < 0.05 vs. PBS group, *P < 0.05, **P < 0.01 vs. positive control group (10 μg/ml CPSIT_P7).