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. 2020 Dec 3;11:578009. doi: 10.3389/fmicb.2020.578009

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Copyright © 2020 Chen, Li, Yan, Sun, Wang, Liu, Xiao, Lu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CPSIT_P7 induced the expression of IL-6, IL-8, and MCP-1 in THP-1 cells via MyD88. THP-1 cells were transfected with 1, 2, or 4 μg of dominant negative plasmid encoding MyD88 (pDeNy-hMyD88) or empty vector (pDeNy-mcs) as a control for 48 h and were treated with CPSIT_P7 (10.0 μg/ml, 24 h) to detect the levels of IL-6, IL-8, and MCP-1 mRNA by RT-qPCR (A–C), or were treated with 10 μg/ml CPSIT_P7 for 36 h to detect the levels of IL-6, IL-8, and MCP-1 protein in the cellular supernatants by ELISA (D–F). The results shown are representative of three independent experiments. Data are means ± SD. *P < 0.05, **P < 0.01 vs. control group (10 μg/ml CPSIT_P7, 0 μg pDeNy-hMyD88).