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. 2020 Dec 16;10:22043. doi: 10.1038/s41598-020-78781-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression level of the transgene (TF-TagRFP) in transgenic hybrid aspens measured by real-time PCR using primer pairs specific to the RFP (A). pGWB560 harboring the NLS-TagRFP was used as a vector control. TagRFP regions fused with each introduced gene as well as vector control were amplified. No amplicon was detected in wild type of T89 plants. Released glucose amounts from biomass (wt/wt %) of seedling stems of the transgenic hybrid aspens grown in MS culture under aseptic condition after 48 h-treatment with enzyme cocktail (B). A series of TFs were sorted by average of released glucose (%) from different lines generated from the same genotype (filled circle).