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. 2020 Dec 16;10:22043. doi: 10.1038/s41598-020-78781-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression level of a total of 29 genes involved in lignin and cellulose biosynthesis in seedling stems of Pt × tERF123ox, and Pt × t ZHD14ox lines grown for one-month in MS culture under aseptic condition, in comparison to the controls (GFP-TUA6). Expressions were measured by real-time PCR by using gene specific primer pairs listed in Table S1, expression levels between samples were normalized by using 18S ribosomal RNA gene expression, and then relative expressions were calculated by using expression levels in control as 1. Data are shown as mean ± standard deviations of biological triplicates. Single and double asterisk(s) indicate P value < 0.05 and < 0.01, respectively in Student’s t- test when compared with the control plants.