Table 2.
Cell wall lignin and polysaccharide analyses of Pt × tERF123ox and Pt × tZHD14ox transgenic hybrid aspens grown in green house.
| Sample | Lignin and sugar content (mg/g CWR) | Lignin aromatic compositiond | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lignina | Arabinan | Xylan | Mannan | Galactan | Amorphous glucanb | Crystalline glucanc | S (%) | G (%) | H (%) | S/G ratio | ||
| Control | 185.4 ± 6.0 | 3.3 ± 0.4 | 116.5 ± 12.8 | 7.0 ± 0.4 | 6.4 ± 0.3 | 9.5 ± 0.7 | 371.7 ± 0.6 | 60.6 ± 2.0 | 39.3 ± 2.0 | Trace | 1.55 ± 0.13 | |
| Pt × tERF123ox line #1 | 168.6 ± 2.2** | 3.3 ± 0.2 | 86.4 ± 4.0* | 6.2 ± 0.6 | 6.5 ± 0.4 | 9.7 ± 0.5 | 383.8 ± 7.6 | 61.3 ± 0.5 | 38.7 ± 0.5 | Trace | 1.65 ± 0.15 | |
| Pt × tERF123ox line #3 | 174.1 ± 7.6 | 4.1 ± 0.7 | 98.5 ± 12.2 | 5.9 ± 0.3* | 7.1 ± 0.5 | 10.3 ± 1.0 | 375.5 ± 7.7 | 60.0 ± 1.1 | 39.9 ± 1.1 | Trace | 1.40 ± 0.05 | |
| Pt × tERF123ox line #8 | 173.6 ± 1.2* | 3.7 ± 0.1 | 96.9 ± 5.0 | 6.9 ± 0.5 | 6.0 ± 1.7 | 9.8 ± 0.4 | 383.5 ± 14.2 | 57.9 ± 0.8 | 42.0 ± 0.8 | Trace | 1.58 ± 0.03 | |
| Pt × tZHD14 line #1 | 179.9 ± 2.7 | 3.3 ± 0.1 | 114.7 ± 7.2 | 5.6 ± 0.6* | 7.3 ± 0.6 | 11.6 ± 0.3** | 382.5 ± 5.7 | 61.8 ± 1.8 | 38.2 ± 1.8 | Trace | 1.51 ± 0.07 | |
| Pt × tZHD14 line #5 | 207.3 ± 5.6** | 4.0 ± 0.1* | 116.4 ± 8.4 | 5.9 ± 0.5* | 7.4 ± 0.1** | 10.8 ± 1.4 | 357.1 ± 2.7** | 58.0 ± 0.8 | 41.5 ± 0.8 | Trace | 1.38 ± 0.05 | |
Single and double asterisk(s) indicate P value < 0.05 and < 0.01, respectively, in Student’s t- test (n ≥ 3) when compared with the control plant.
CWR cell wall residues, S syringyl, G guaiacyl, H p-hydroxyphenyl.
aDetermined by thioglycolic acid assay.
bTrifluoroacetic-acid-soluble glucan.
cTrifluoroacetic-acid-insoluble glucan.
dDetermined by analytical thioacidolysis.