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. 2020 Dec 16;10:22047. doi: 10.1038/s41598-020-78987-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of CHIR99021 and DAPT on myogenic differentiation of iMyoD-hTERT-NHDFs in 2D culture. (A) Illustration of cell culture regimens. (B) Cell morphology after 2 weeks of culture as revealed by immunofluorescent staining for total MHC (green) and counterstaining for nuclei (blue). (C–E) Quantification of myogenic index, myotube width, and myotube length. The experiments were conducted in triplicate with 3 independent samples. Data are presented as mean ± SEM. Statistical analysis was performed by one-way ANOVA with Tukey’s test. **p < 0.01, ***p < 0.001.