Figure 3.

Gene expression and western blot analyses for iMyoD-hTERT-NHDFs cultured using the culture regimens illustrated in Fig. 2A. (A) Relative gene expression analysis for myogenic markers. The expression levels were normalized to a housekeeping gene β-actin. The experiments were conducted in triplicate with 3 independent samples. Data are presented as mean ± SEM. Statistical analysis was performed by one-way ANOVA with Tukey’s test. *p < 0.05, **p < 0.01. (B) Western blot analysis of total MHC and neonatal MHC. Actin was used as a housekeeping protein. Full-length blots are presented in Figure S5.