Table 1A.

Overview of the diagnostic workup of the three cases of feline tuberculosis.

Case	Geographical origin	Symptoms/lesions	Radiology	FNA	Histopathology	Bacteriology	IGRA (BoPPD/ AvPPD)
1	Märstetten, Thurgau	Generalized enlarged LN, multiple subdermal skin nodules	n/a	LN: numerous (60%) mature lymphocytes and moderate (20%) neutrophils partly degenerated	Skin nodule: high degree pyogranulomatous panniculitis; LN: lymphadenitis, no AFB by ZN staining	AFB in ZN staining, positive MTBC real-time PCR, M. microti in culture (6 weeks)	0.50/0.246
2	Wolfhausen, Zurich, originally from eastern Europe	Enlarged mandibular LN, respiratory symptoms	Radiography: severe increase of pulmonary opacity, severe bronchial-interstitial pattern; CT: severe, generalized increase in pulmonary attenuation	LN: activated macrophages indicating a pyogranulomatous inflammation (low cellularity of the smear)	LN and lung: granulomatous inflammation with intralesional AFB	Curved AFB in ZN staining, positive MTBC real-time PCR, M. microti in culture (4 weeks)	n/a
3	Fieschertal, Valais	Enlarged mandibular LN, ulcerative skin lesions	CT: severe mandibular and retropharyngeal lymphadenopathy, miliary pulmonary pattern	LN: numerous lymphatic cells, mainly small lymphocytes and moderate (10–20%) neutrophils without degeneration	LN: massive chronic granulomatous to pyogranulomatous lymphadenitis, no AFB by ZN staining	Curved AFB in ZN staining, positive MTBC real-time PCR, bacterial species identification by HRM, no growth of M. microti	7.82/2.96

LN, lymph nodes; AFB, acid fast bacilli.