TABLE 2.

Fatty alcohol production in E. coli.

Properties	Host strain	Product-forming enzyme	Mutations	Media	Shake flask titer (mg L–1)	Fed batch titer (mg L–1)	Productivity (mg L–1h–1)	Yield (g/g)	References
Even chain C12–C18	C41 (DE3)	AcACR1	‘tesA, fadD, ΔfadE	M9	60	ND	ND	ND	Steen et al., 2010
	BL21 (DE3)	AbACR1	BTE, fadD	M9	37.5	598	24.94	0.001	Zheng et al., 2012
	MG1655	MaFAR	3X BTE, FadD	M9	ND	1650	13.75	0.134	Youngquist et al., 2013
	BL21(DE3)	MaFAR	‘tesA, fadD	M9	422	1750	16	0.028	Liu et al., 2013
	BL21 (DE3)	MmCAR	‘tesA, AHR, Bssfp	Minimal	350	ND	ND	0.05	Akhtar et al., 2013
	MG1655	MaFAR	fadD, ‘tesA, ΔfadE, araD:acpS (C. glutamicum)	M9	3500	ND	50	0.126	Haushalter et al., 2015
	DH5α	SeAAR	ybbO, fadR, ΔplsX, fabZ	M9	270	1989	41.43	ND	Fatma et al., 2016
	MG1655	MaFAR	ΔtesCB, ΔldhA, Δpta, ΔackA	LB	760	6330	126	ND	Liu et al., 2016
	MG1655	MaFAR	ΔfadE	M9 (2mM NH4+)	180	ND	ND	0.12	Chubukov et al., 2017
	DH5α	SeAAR	zwf, ybbO, Δedd, Δpps, ΔldhA, ΔaceA, ΔpoxB, Δpta, ΔpflB, ΔplsX	Modified mineral medium	1506	12500	ND	ND	Fatma et al., 2018
Octanol	MG1655	MaFAR	ΔaraBAD, ΔfadE, ΔfadD::PTRCCpFatB1*-lacI, ΔackA-pta::PTRC-Mt-FadD6–lacI, ΔpoxB::PTRC-Mt-FadD6, ΔFadBA::PTRCMa-ACR-lacI	Mineral medium 2% glycerol	1273		53	0.064	Hernandez Lozada et al., 2020
Octanol	BL21 (DE3)	Mm CAR	BsSFP, At tes3 AHR	LB	62		4.4	12	Akhtar et al., 2015
Odd (C2n–1) chain	BL21 (DE3)	O. sativa αDOX	‘tesA FadR, AHR	M9 yeast extract, glycerol	105	1950	105	0.018	Cao et al., 2015
Branched chain	UB1005	MaFAR	ΔfabH, ΔfadE, ΔldhA, fadR, ilvCD, S. aureus fabH, B. subtilis lplA, leu operon, and bkd operon	M9	170	350 branched chain, 737 total fatty alcohol	9	ND	Jiang et al., 2017