TABLE 4.

Fatty alcohol production (C16 and C18) in S. cerevisiae.

Background strain	Enzyme	Genotype (strain name)	Titer (shake flask) mg L–1	Titer (fed batch) mg L–1	Productivity (mg L–1h–1)	Yield (g/g) carbon	References
BY4742	MmFAR1	pESC-PGAL1-mFAR1	47.4	N/A	ND	0.002	Runguphan and Keasling, 2014
BY4742	MmFAR1	acc1::PTEF1-ACC1; fas1::PTEF1-FAS1; fas2:: PTEF1-FAS2, pESC-PGAL1-mFAR1 (WRY1)	93.4	N/A	ND	0.005	Runguphan and Keasling, 2014
BY4742	MmFAR1	acc1::PTEF1-ACC1; fas1::PTEF1-FAS1; fas2:: PTEF1-FAS2, pESC-PGAL1-m FAR1/PGAL10-MaME	98	N/A	ND	0.005	Runguphan and Keasling, 2014
BY4741	TaFAR1	dga1::HIS3; pYES2 -PGAL1-TaFAR1	73	N/A	ND	0.004	Tang and Chen, 2015
BY4742	TaFAR1	rpd3Δ; pRS425-PTEF1-TaFAR-TTEF1	122.4	N/A	ND	ND	Feng et al., 2015
BY4742	TaFAR1	rpd3Δ; pRS425-PTEF1-TaFAR-TTEF1-PPGK1-ACC1-THXT7 pRS423-PTPI1-YlACL1-TTPI1-PTEF1-YlACL2-TTEF1 (XF3)	330.2	1111	42	0.03*	Feng et al., 2015
WRY1	MmFAR1	1622b::PGAL1-MmFAR1-TTDH1 208a::PTEF1-MmFAR1-TCYC1 YPRCd15c::PGAL1-ACC1**-TENO2; hfd1Δ adh6Δ gdh1Δ dga1Δ 1014a::PTEF2-OLE1-TADH1 gal80Δ::PTDH3-GAL4 (yL434)	1200	6000	ND	0.06	d’Espaux et al., 2017
CEN.PK113-11C	MaFAR	pox1Δ are1Δ are2Δ lro1Δ dga1Δ; pYX212-(PTPI1-MaFARper2-pYX212t)	193	N/A	ND	0.01	Zhou et al., 2016a
CEN.PK113-11C	MmCAR & MaFAR	(FOH33) hfd1Δ pox1Δ faa1Δ faa4Δ, adh6Δ::kanMX, gal80Δ, gal1/10/7Δ::(PGAL7-MmCAR-TADH1)+(PGAL3-npgA-TFBA1); pYX212-(PTPI1-npgA-TFBA1)+(PTDH3-MmCAR-TADH1)+(PHXT7-ADH5-TCYC1)+(PTEF1-MaFAR-pYX212t))	120	1500	ND	0.006	Zhou et al., 2016b

*Actual yields and productivity are not clear owing to the high density and initial fatty alcohol content of the starting culture. **A hyperactive ACC1 mutant. ND, Not Determined. N/A, Not Applicable.