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. 2020 Dec 3;8:601953. doi: 10.3389/fcell.2020.601953

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Copyright © 2020 Wang, Xu, Xie, Hu, Jiang, Hu, Ding, Xiao and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ASS promoted exosomes and autophagy related components release. (A,B) A549 cells derived exosomes observed by TEM, Scale bar, 200 nm. (C) The intensity and size of exosomes as evaluated by NANOSIGHT. (D,E) The concentration of exosomes isolated from A549 and H1299 cells after exposure to ASS for 60 min as acquired by Nanoparticle Tracking Analysis (NTA), ***P < 0.001. (F–K) Expression of exosomes maker (TSG101, CD63, and HSP70) and autophagy component maker (LC3I/II) were measured by western blot analysis, every experiment was repeated in triplicate, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.