FIGURE 3.

Suppression of exosomes release exacerbated ASS induced NSCLC cell apoptosis. (A) A549 and H1299 cells were pretreated with exosome inhibitor GW4689 (GW) for 60 min and then exposed to ASS (10 dyn/cm²) for 60 min, the concentration of isolated exosomes were detected by NTA. (B) The expression of exosomes marker (TSG101, CD63, and HSP70) and secreted autophagy components (LC3 I/II) were analyzed by western blot. (C–F) After pretreatment with GW4689 for 60 min, NSCLC cells were exposed to ASS (10 dyn/cm²) for 0, 15, 30, and 60 min, the percentage of cell apoptosis was evaluated by (C,D) TUNEL assay and (E,F) flow cytometry using Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Staining/Detection Kit, *P < 0.05, ***P < 0.001, ****P < 0.0001.