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. 2020 Dec 3;8:601953. doi: 10.3389/fcell.2020.601953

FIGURE 6.

FIGURE 6

SIRT2 promoted TFEB expression through direct binding with TFEB mRNA. (A) Prediction of the interaction between SIRT2 and TFEB 3′ UTR indicated as a heat-map, catRAPID identified the interaction between SIRT2 and TFEB with confidence (Interaction Propensity = 84, Discriminative Power = 98%). (B) Based on data from TCGA, Pearson correlation of SIRT2 and TFEB (P < 0.0001, R = 0.41). (C) A549 cells were either stably knocked-down or overexpressed for SIRT2, and then exposed to ASS (0 and 10 dyn/cm2) for 60 min, the expression of TFEB was determined by western blot, ***P < 0.001 compared to sh-NC group, $$$ P < 0.001 compared to OE-NC group. (D,E) Cellular extracts of NSCLC cells with either stable knockdown or overexpression of SIRT2 were immunoprecipitated with SIRT2 and IgG antibodies, the target TFEB mRNA of the immunoprecipitation was assessed through qRT-PCR and semi-quantitative RT-PCR. *P < 0.05, ****P < 0.0001.