FIGURE 7.

SIRT2 along with TFEB regulates autolysosome formation, exosomes, autophagy component release, cell apoptosis, and invasion. (A,B) NSCLC cells transfected with OE-SIRT2, OE-TFEB, or OE-SIRT2/sh-TFEB plasmid were exposed to ASS (10 dyn/cm²) for 60 min, exosomes which were released were collected and exosome specific markers were detected by western blot, ****P < 0.0001 compared to pcDNA3.1 group, &&&, &&&& denote P < 0.001 and P < 0.0001 compared to shNC group. (C) After treatment as previously described, immunofluorescence was performed to detect the fluorescence intensity of LAMP1 and LC3B, the co-localization of LAMP1 and LC3B was observed as yellow region in the merged image, magnification, 600×. (D,E) The percentage of cell apoptosis was determined by TUNEL assay and flow cytometry, *, **, *** and **** denotes P < 0.05, P < 0.01, P < 0.001, and P < 0.0001 compared to pcDNA3.1 group, &&, &&& denote P < 0.01 and P < 0.001 compared to SIRT2/shNC group. (F) After treatment as previously described, cell invasion was measured by Tranwell assay.