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. 2020 Dec 3;11:548268. doi: 10.3389/fgene.2020.548268

FIGURE 5.

FIGURE 5

MicroRNAs represses the expression of TRIB3 via binding the 3’UTR target sequence. Luciferase activity in HEK293 cells co-transfected with miRNA mimic, miRNA inhibitor, miRNA control and empty vector for the TRIB3 3’UTR. Luciferase activity was assayed 24 h after transfection. All luciferase values were normalized to Renilla luciferase. Blue columns represent the luciferase activity co-transfected with miRNA mimic control; Red columns represent the luciferase activity co-transfected with miRNA inhibitor control; Green columns represent the luciferase activity co-transfected with miRNA mimic; Pink columns represent the luciferase activity co-transfected with miRNA inhibitor. (A) Represents the luciferase activity of TRIB3 after over- or down-expressed miR-2904 compared with controls. (B) Represents the luciferase activity of TRIB3 after transfecting mutant vector of miR-2904 compared with control. *Significant difference between the control and the treatment; **Very significant difference between the control and the treatment.