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. 2020 Oct 22;23:27–41. doi: 10.1016/j.omtn.2020.10.031

Figure 4.

Figure 4

ALKBH5 Was Correlated with CK2α Expression in Bladder Cancer Tissues and Cell Lines

(A and B) Western blot analysis of CK2α, GLUT, HK1, LDHA, LDHB, PKM, and GAPDH in 5637 and T24 cells with ALKBH5 knockdown or overexpression. (C–E) Knockdown of ALKBH5 expression increased uptake of glucose, lactate production, and the intracellular ATP level within 24 h. Data represent the mean ± SD from three independent experiments, ∗p < 0.05. (F–H) Overexpression of ALKBH5 expression suppressed the uptake of glucose, lactate production, and the intracellular ATP level. Data represent the mean ± SD from three independent experiments, ∗p < 0.05. (I) CK2α was expressed higher in bladder cancer tissues than in adjacent normal tissues by qRT-PCR (n = 28, p < 0.001). (J and K) A negative correlation between the expression of CK2α and ALKBH5 was shown in bladder cancer tissues by qRT-PCR and IHC. Scale bars, 100 μm.