Figure 7.

ALKBH5 Inhibited Tumorigenesis In Vivo

(A) Subcutaneous xenograft tumor model with ALKBH5 overexpression (ALKBH5) or control cells (NC). (B) Tumor volume was measured at the indicated weeks after transplanting. Data represent the mean ± SD, ∗p < 0.05. (C) Tumor weight was measured after 3 weeks from transplanting. Data represent the mean ± SD, ∗p < 0.05. (D) Western blot and qRT-PCR analysis of CK2α and ALKBH5 expression in the tumor model. Data represent the mean ± SD, ∗p < 0.05. (E) IHC analysis showed that the expression of CK2α and cell proliferation marker Ki67 decreased in ALKBH5. Data represent the mean ± SD from three independent experiments, ∗p < 0.05 (F) Working model: ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2α-mediated glycolysis.