Supplementary Fig. S3.

Root mean square deviation (RMSD) graph by 40 ns simulation. All were plotted against native P53 (Black). a) 8 Benign/Likely Benign variants; b) 38 Pathogenic variants; and c) 42 VUS. The average of RMSD is 0.320 nm for native P53, 0.348 for Benign/ Likely Benign and 0.327 nm for VUS. Pathogenic mutants were separated into 2 regions, the higher (Y163C, R175H, Y220C, G245D, G245S, R248Q, R273C, R282W) and the lower region with 0.672 ± 0.069 nm and 0.327 ± 0.050 nm, respectively. Only R249S in VUS was identified to have deleterious effects on protein structure, but not for other VUS. The figure showed that RMSD can only identify the variants with significant deleterious effects on protein structure.