FIGURE 6.
Napabucasin inhibits HCC homograft growth in vivo. (A) C57BL/6J mice were inoculated with 5 × 106 Hepa1-6 cells in the left axilla, and intraperitoneally injected with napabucasin (20 mg/kg) or vehicle 5 days post-inoculation every 2 days for a total of eight injections. (B) Representative images of tumor tissues. (C) The percentage of tumor free mice. (D) Tumor weight of the suitably treated mice. (E) Stemness marker expression in the tumor cells. Data were normalized to β-actin. (F) Frequency of Ki67+ cells in the tumors. (G) Ki67 levels in tumor cells from mice treated as above. MFI, mean fluorescence index. Data represents the mean ± SD of n = 5 and were analyzed with one-way ANOVA (*P < 0.05, **P < 0.01 and ***P < 0.001).