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Verification of UBA1 levels in HCC cells. (A, B) UBA1 expression in some HCC cell lines was detected by qRT–PCR and Western blotting. (C, D) Effects of UBA1 interference within Huh7 cells at the mRNA or protein level. (E) Cell proliferation ability detected by CCK-8 viability following UBA1 expression interference. (*p < 0.05; **p < 0.01; ##p < 0.01).
