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. 2020 Dec 3;10:592413. doi: 10.3389/fonc.2020.592413

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Copyright © 2020 Shan, Yang, Huang, Zhou, Hu, Lu, Guo, Hou, Cao, Tian and Pan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of changed UBA1 expression on malignant phenotypes and ferroptosis in HCC cells. (A) Wound healing assay. Migratory cells to the wound were determined following different treatments and imaged. (B, C) Transwell migration and invasion assays. Huh7 cells treated with different drug doses were analyzed for their invasion and migration capacities. (D) Ferrous iron and the MDA content (E) in Huh7 cells subjected to si-UBA1 transfection (* si-UBA1 versus si-UBA1+oltipraz, **p < 0.01, *p < 0.05; # si-NC versus si-UBA1, ^##p < 0.01, ^#p < 0.05).