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. 2020 Dec 3;11:607161. doi: 10.3389/fpls.2020.607161

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Copyright © 2020 Maioli, Gianoglio, Moglia, Acquadro, Valentino, Milani, Prohens, Orzaez, Granell, Lanteri and Comino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genotyping of targeted gene mutations induced by CRISPR/Cas9 in the T₁ and T₂ generations. (A) Mutagenesis frequencies for all three targeted loci in T₁ and T₂ progenies. (B) Zygosity of targeted gene mutations in T₁ and T₂ populations.