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. 2020 Dec 16;10:22023. doi: 10.1038/s41598-020-79146-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of anemia in Dahl/SS rats. Comparison of hemoglobin (a), log-transformed serum erythropoietin (b), Fe (c), and total iron binding capacity (TIBC) (d) levels in the control (n = 7) and the HS (n = 8) groups at 15–17 weeks of age. The number (e) and ratio (f) of reticulocyte in the control (n = 7) and the HS (n = 9) groups. Supravital staining image of reticulocytes by new methylene blue (bars 10 μm) in the control and the HS groups (g). Statistical analysis was performed using unpaired Student’s t test by JMP Pro Version 14.2.0 software (SAS Institute Inc.). *p < 0.05 vs. the control group.