Figure 2.
circPHIP inhibits the migration, invasion, and proliferation of OSCC
(A) After stable transfection of sh-circPHIP or negative control (NC) vector, circPHIP expression levels in SCC15 and CAL27 cells were detected using qRT-PCR. (B) Transwell migration and invasion assays in stable SCC15 and CAL27 cells with circPHIP knockdown. (C) Wound-healing assays in stable SCC15 and CAL27 cells with circPHIP knockdown. (D) Colony-formation assays in stable SCC15 and CAL27 cells with circPHIP knockdown. (E) CCK-8 assays were performed in stable SCC15 and CAL27 cells with circPHIP knockdown. (F) EdU incorporation assays in stable SCC15 and CAL27 cells with circPHIP knockdown. (G) Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining of stable SCC15 and CAL27 cells with circPHIP knockdown. The percentage of apoptotic cells is shown. All data are presented as mean ± SEM of three independent experiments. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Representative images are shown. Scale bars, 20 μm.