Figure 3.

circPHIP acts as an miR-142-5p sponge in OSCC cells

(A) RIP assay was performed to detect circPHIP levels in HOKs transfected with AGO2. (B) Schematic exhibiting the overlap in circPHIP target miRNAs predicted by the miRTarBase, miRDB, mirDIP, and TargetScanHuman databases. (C) Relative miR-142-5p levels in SCC15 and CAL27 lysates. (D) Predicted circPHIP and miR-142-5p binding sites. (E) Luciferase activities of HOKs cotransfected with a luciferase reporter construct containing wild-type (WT) or mutant (Mut) circPHIP and miR-142-5p or NC mimic. All data are presented as mean ± SEM of three independent experiments. Student’s t test, ∗∗p < 0.01, ∗∗∗p < 0.001.