Figure 7.

PHIP and ACTN4 function as driver genes in OSCC cells

(A) Overexpression of PHIP and ACTN4 in SCC15 and CAL27 cells with stable depletion of circPHIP, and the levels of indicated proteins were determined via western blotting. (B) Transwell migration and invasion assays were performed in SCC15 and CAL27 cells with stable depletion of circPHIP and PHIP or ACTN4 overexpression. (C) Wound-healing assays were performed in SCC15 and CAL27 cells with stable depletion of circPHIP and PHIP or ACTN4 overexpression. (D) CCK-8 assays were performed in SCC15 and CAL27 cells with stable depletion of circPHIP and PHIP or ACTN4 overexpression. (E) Colony-formation assays were performed in SCC15 and CAL27 cells with stable depletion of circPHIP and PHIP/ACTN4 overexpression. (F) Apoptosis assays were performed in SCC15 and CAL27 cells stably depleting circPHIP and PHIP or ACTN4 overexpression. All data are presented as mean ± SEM of three independent experiments. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Representative images are shown. Scale bars, 20 μm.