Figure 8.
circPHIP functions as an miR-142-5p sponge to promote tumorigenesis in vivo
(A) Nude mice were subcutaneously injected with 5 × 106 SCC15 cells or cells stably expressing sh-circPHIP or with the miR-142-5p inhibitor (n = 8 per group). (B) Tumor weights from euthanized mice. (C) Weekly measurements of tumor volumes were recorded every 6 days starting the day mice were injected with SCC15 cells stably expressing sh-circPHIP. (D) The indicated protein levels in tumors from the different groups were analyzed via western blotting. (E) H&E and IHC staining were performed using OSCC xenografts from the different groups. All data are presented as mean ± SEM. Student’s t test, ∗∗∗p < 0.001. Representative images are shown. Scale bars, 100 μm.