Figure 3.

AZ can exert its antiviral effect independently of the activation of interferon pathway. A, The relative infection rate of IAV‐luc in WT or IFNAR1 KO HEK293T cells 24 h post‐infection. B, The relative inhibitory rate of IAV‐luc infection in WT or IFNAR1 KO HEK293T cells treated by IFN‐beta (10 ng/mL) or AZ (10 μmol/L) 24 h post‐infection. C, The relative inhibitory rate of IAV‐luc infection in WT or RIG‐I KO HEK293T cells treated by AZ (2.5 μmol/L) 24 h post‐infection. D‐F, The relative mRNA level ofIFNB1,ISG54andCCL5in A549 cells treated with ethanol or AZ (2, 10, 50 μmol/L) and stimulated with WSN for 12 h. Solvent was treated as Ctrl. All results are representative of three replicate experiments. ns, no significant, *P < .05, **P < .01, ***P < .001, ****P < .0001